Fig. 2

Critical regions in the predicted amino acid sequence of starch branching enzymes (SBEs) in the catalytic A domain in horticultural crops. The conserved SBE1 and 2 residues are invariant but the residues in the SBE3 isoform contain many substitutions. The four critical regions located within the central A-catalytic domain (black area in the middle of SBE protein) were assigned as Regions 1 to 4, respectively. The Regions within the SBE3 are less conserved than those in SBE1 and SBE2. A chloroplast transit peptide (cTP), a carbohydrate-binding module family 48 domain (CBM_48), and an α-amylase C-terminus (AMY_C) are shown. The small black bars on the x-axis indicate the catalytic/active residues, while the white bars represent variant residues at those sites. The Y-axis of each logo shows the probability of residues present on that specific site from the species listed in the Fig. 1. Residue logos were generated from WebLogo3 [70], and the figure was made in Microsoft® PowerPoint