Fig. 4

Optimization of the pOp6/LhGR induction in rice by testing different steroid inducers. A Shoots of 10-day-old seedlings (lines 65A, 121C) were induced in water supplemented with either 0.01% or 0.1% Tween-20 and 30 μM steroid inducer Dex, deoxycorticosterone (Doc) or triamcinolone acetonide (TA) for 24 h, then histochemically stained for GUS activity. B Leaf cuttings of the same leaf of 6-week-old plants (lines 65A, 121C) were induced in water supplemented with 0.1% Tween-20 and 10 μM Dex or 10 μM TA for 24 h, then histochemically stained for GUS activity. Leaves of pZmUbi::GUS were included as controls. Representative images are shown. C Leaves of 5-week-old plants (lines 65A top, 121C bottom) were induced by painting with water supplemented with 0.1% Tween-20 and 30 μM steroid inducer: Dex, betamethasone (Bet), fludrocortisone acetate (Flu), prednisone (Pre) or prednisolone (Plo) for 24 h, then histochemically stained for GUS activity