Table 1 Summary of Illumina sequencing data and mapped sequence reads for the assayed alfalfa samples
Genotype | Tissue | Treatment | Biological replicate | Total reads | Mapped reads | Mapping rate (%) |
|---|---|---|---|---|---|---|
Halo | Leaf | Control | 1 | 7,235,661 | 6,275,026 | 86.7 |
Control | 2 | 7,462,991 | 6,607,722 | 88.5 | ||
Control | 3 | 7,532,603 | 6,371,474 | 84.6 | ||
Control | 4 | 7,163,647 | 6,286,906 | 87.8 | ||
Stressed (3h) | 1 | 7,170,087 | 6,291,489 | 87.7 | ||
Stressed (3h) | 2 | 8,619,083 | 7,531,679 | 87.4 | ||
Stressed (3h) | 3 | 7,275,223 | 6,453,594 | 88.7 | ||
Stressed (3h) | 4 | 7,346,150 | 6,417,574 | 87.4 | ||
Stressed (27h) | 1 | 7,234,036 | 5,968,374 | 82.5 | ||
Stressed (27h) | 2 | 7,186,154 | 6,225,839 | 86.6 | ||
Stressed (27h) | 3 | 6,256,894 | 5,471,745 | 87.5 | ||
Stressed (27h) | 4 | 5,696,229 | 4,741,642 | 83.2 | ||
Root | Control | 1 | 7,930,008 | 6,833,311 | 86.2 | |
Control | 2 | 7,568,654 | 6,104,814 | 80.7 | ||
Control | 3 | 10,017,590 | 9,054,027 | 90.4 | ||
Control | 4 | 6,523,142 | 5,561,719 | 85.3 | ||
Stressed (3h) | 1 | 9,003,316 | 7,662,274 | 85.1 | ||
Stressed (3h) | 2 | 11,023,879 | 9,201,753 | 83.5 | ||
Stressed (3h) | 3 | 9,647,653 | 8,529,106 | 88.4 | ||
Stressed (3h) | 4 | 6,201,499 | 4,884,633 | 78.8 | ||
Stressed (27h) | 1 | 6,563,910 | 4,983,668 | 75.9 | ||
Stressed (27h) | 2 | 8,321,924 | 3,727,203 | 44.8 | ||
Stressed (27h) | 3 | 9,424,651 | 7,313,948 | 77.6 | ||
Stressed (27h) | 4 | 7,942,407 | 2,854,933 | 35.9 | ||
Vernal | Leaf | Control | 1 | 7,085,736 | 6,147,440 | 86.8 |
Control | 2 | 7,149,929 | 6,449,664 | 90.2 | ||
Control | 3 | 8,108,009 | 7,362,827 | 90.8 | ||
Control | 4 | 10,775,421 | 9,953,409 | 92.4 | ||
Stressed (3h) | 1 | 5,372,062 | 4,901,710 | 91.2 | ||
Stressed (3h) | 2 | 6,180,723 | 5,477,930 | 88.6 | ||
Stressed (3h) | 3 | 7,355,464 | 6,390,995 | 86.9 | ||
Stressed (3h) | 4 | 6,775,443 | 6,098,490 | 90.0 | ||
Stressed (27h) | 1 | 11,398,645 | 10,388,765 | 91.1 | ||
Stressed (27h) | 2 | 7,517,258 | 6,754,908 | 89.9 | ||
Stressed (27h) | 3 | 8,128,644 | 7,280,110 | 89.6 | ||
Stressed (27h) | 4 | 7,713,476 | 7,007,553 | 90.8 | ||
Root | Control | 1 | 11,004,685 | 9,744,748 | 88.6 | |
Control | 2 | 10,402,513 | 9,091,041 | 87.4 | ||
Control | 3 | 8,070,744 | 7,001,864 | 86.8 | ||
Control | 4 | 8,936,244 | 7,616,107 | 85.2 | ||
Stressed (3h) | 1 | 8,293,827 | 7,159,751 | 86.3 | ||
Stressed (3h) | 2 | 8,722,568 | 7,705,076 | 88.3 | ||
Stressed (3h) | 3 | 9,769,559 | 8,854,805 | 90.6 | ||
Stressed (3h) | 4 | 6,402,547 | 5,317,429 | 83.1 | ||
Stressed (27h) | 1 | 3,669,213 | 3,121,348 | 85.1 | ||
Stressed (27h) | 2 | 9,000,614 | 7,858,878 | 87.3 | ||
Stressed (27h) | 3 | 8,661,229 | 7,569,795 | 87.4 | ||
Stressed (27h) | 4 | 8,640,454 | 7,481,651 | 86.6 | ||
Total | 381,482,398 | 324,090,747 | Â | |||
Average (Control) | 8,310,474 | 7,278,881 | 87.4 | |||
Average (3 h) | 7,882,443 | 6,804,893 | 87.0 | |||
Average (27 h) | 7,709,734 | 6,171,898 | 80.1 | |||
Average | 7,947,550 | 6,751,891 | 84.8 | |||