Fig. 1
V14 is critical to leaf development in the early stages of seedling. A V14 deficiency in the v14 mutant demonstrated by immunoblotting analysis of the first and third leaves of v14. B Chloroplast localization of V14 indicated by the green fluorescence expressed by V14-fused EGFP in chloroplasts. BF, bright field; Chl, autofluorescence of chlorophyll (red); Bar = 10 μM. C Expression of V14 in different organs and at different stages of development. Coty, cotyledon; L1-L5, the first to the fifth true leaf; FL, flag leaf. D Complementation of the v14 mutant confirmed by phenotypic analysis and V14- and v14-specific PCR in T1 plants. G, green; v, virescent. The un-cropped gel image is provided in Additional file 1 B. Bar = 0.5 cm (E) Reproduction of the v14-like phenotype in the T1 generation of two independent V14-RNAi lines, which is confirmed by phenotypic analyses and the gene expression analysis of V14 by qRT-PCR. Bar = 0.5 cm. The significant difference between T+ (with the transgene) and T- (without the transgene) plants was analyzed by student’s t test (n = 3). *, P < 0.05; **, P < 0.01. (A) and (B), The data presented here are the representative images of three independent experiments. (C) and (E), The relative expression levels shown here are the averages of three independent experiments