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Fig. 7 | BMC Plant Biology

Fig. 7

From: Construction of a high-density genetic map and detection of a major QTL of resistance to powdery mildew (Erysiphe necator Sch.) in Caucasian grapes (Vitis vinifera L.)

Fig. 7

Summary of the conidia classification in Trypan-Blue bioassays. The images show the E. necator conidia development at 2 dpi on ‘Shavtsitska’ (a) and ‘Glera’ (b). In ‘Shavtsitska”, the pathogen growth was delayed and more limited than in ‘Glera’, furthermore plant necrotic response-HR (brown area) was present under the conidia appressoria (⎈) and under hyphae appressoria (▲) (magnification 200x and scale bar 100 μm). Below the conidia classification at 1-2-3 dpi following the Trypan-Blue staining (c). Class 0 (C0 - red) identifies conidia showing the appressoria, class 1 (C1 - blue) the conidia with the primary hypha, class 2 (C2 - green) the conidia with the secondary hypha and class 3 (C3 - violet) the conidia with the tertiary hypha and/or hyphae ramification. Seedlings ‘7010Z’, ‘7116Z’ and ‘7371Z’ are resistant offspring while ‘7008Z’, ‘7017Z’ and ‘7190Z’ are susceptible ones

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