Fig. 8

Col 35S::LbHLH lines exhibit increased salt tolerance. A The expression patterns of LbHLH in L. bicolor after 100 mM NaCl treatment for different periods of time (0, 12, 24, 48, and 72 h); the 0 h NaCl treatment was used as the control (relative expression level = 1). B Seeds of all lines were first germinated on the 1/2MS basic medium for 4 days before transferred to different NaCl treatments (0, 50, 100, and 150 mM) for 5 days. C Analysis of germination and seedling growth under different NaCl treatments. Germination percentage was measured at 48 h and 5 days after sowing. Thirty seeds per line were sown per treatment, and three replicates were performed. Germination percentage data are the mean ± SD of three replicates. The cotyledon growth rate (expressed as the percentage of plants with emerged cotyledons) was calculated 3 days after sowing on different media. Thirty seeds per line were sown per treatment, and three replicates were performed. Cotyledon growth rate data are mean ± SD of three replicates. Root lengths of nine-day-old seedlings of (B) were determined using ImageJ software. Thirty seedlings were analyzed per line. Root length data are the mean ± SD of 30 plants. Different letters indicate significant differences at P = 0.05 according to Duncan’s multiple range test