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Fig. 7 | BMC Plant Biology

Fig. 7

From: A novel gene LbHLH from the halophyte Limonium bicolor enhances salt tolerance via reducing root hair development and enhancing osmotic resistance

Fig. 7

Yeast two-hybrid assay of the interaction between LbHLH and AtGL1 or AtGL3. A Verification of self-activation and interactions in Y2H Gold yeast. AD + 53: AD-T + BD-53 (positive control); AD + Lam: AD-T + BD-Lam (negative control); AtGL1 + BD: AD-AtGL1 + BD (test of AtGL1 self-activation); AtGL3 + BD: AD-AtGL3 + BD (test of AtGL3 self-activation); LbHLH + AD: BD-LbHLH + AD (test of LbHLH self-activation); AtGL1 + LbHLH: AD-AtGL1 + BD-LbHLH (test of Interaction 1); AtGL3 + LbHLH: AD-AtGL3 + BD-LbHLH (test of Interaction 2). B Yeast transformation on SD/-Leu/-Trp medium. C Tests of self-activation and interactions on SD/-Leu/-Trp/X-a-gal/Aba (200 ng/ml) and SD/-Ade/-His/-Leu/-Trp/X-a-gal/Aba (200 ng/ml) media

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BMC Plant Biology

ISSN: 1471-2229

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