Fig. 10

LbHLH enhances salt tolerance by enhancing the ability to resist osmotic stress. A The seeds of all lines were first germinated on the 1/2MS basic medium for 4 days before transferred to different media containing 180 mM sorbitol, 10 mM LiCl, or 100 mM NaCl for 5 days. B Germination percentage calculated each day after sowing on different media. Thirty seeds per line were sown per treatment, and three replicates were performed. Data are the mean ± SD of three replicates. C Root lengths of (A) were determined using ImageJ software. Thirty seedlings were analyzed per line. Data are the mean ± SD of 30 plants; different letters indicate significant differences at P = 0.05 according to Duncan’s multiple range test