Fig. 6

Representative fluorescence microscopic images of in vivo detection of hydrogen peroxide (H₂O₂), superoxide anion (\( {\mathrm{O}}_2^{\cdotp -} \)) in leaves of cotton plants treated with different chemical compounds. (A) H₂O₂ detected with the DCF-DA fluorescence probe (green color) and its corresponding bright field (lower panels). (B) \( {\mathrm{O}}_2^{\cdotp -} \) detected with 10 μM DHE (red color) and its corresponding bright field (lower panels). The leaves of seedlings were treated with control (CK), salt-treated (S), S + MT200 for 72 h. Control (CK) and salt-treated (S) plants were sprayed with distilled water, while S + MT200 was sprayed with 200 μM MT