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Fig. 5 | BMC Plant Biology

Fig. 5

From: Evolutionary analysis and functional characterization of SiBRI1 as a Brassinosteroid receptor gene in foxtail millet

Fig. 5

SiBRI1 regulated the BR response in foxtail millet. (A), Phenotype of light-grown 4-leaf stage Ci846 plants and two independent lines of pUbi:SiBRI1-eGFP/Ci846 (SiBRI1-OX); scale bar = 1.5 cm. (B), Expression levels of SiBRI1-YFP in the transgenic plants shown in (A). Ponceau S staining of the Rubisco large subunit was used as an equal loading control. (C), Quantitative real-time RT-PCR analysis of SiCPD, SiD2, SiDWARF and SiDWF4 expression in the roots of 8-day-old seedlings overexpressing SiBRI1 under 1 μM BL immersion for 1 h. Three biological repetitions were established. Error bars indicate the mean ± standard deviation (SD). Statistically significant differences are indicated by different lowercase letters (p < 0.05, one-way ANOVA with Tukey’s significant difference test)

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