Fig. 3

SiBRI1 overexpression rescued the mutant phenotypes of bri1-116 plants. (A), Phenotypes of light-grown 8-week-old Col-0 and bri1-116 mutant plants overexpressing SiBRI1 with a C-terminal YFP tag. An enlarged view of bri1-116 is shown in the white box. Scale bar = 1 cm. (B), Expression levels of SiBRI1-YFP and AtBZR1 in the transgenic plants shown in (A). The differential accumulation pattern of AtBZR1 in (A) was detected by anti-BZR1; pAtBZR1 showed the phosphorylation form of AtBZR1, and AtBZR1 showed the unphosphorylated form of AtBZR1. Anti-HSP70 and Ponceau S staining of the Rubisco large subunit was used as an equal loading control. The bottom gel shows the genotyping identification of the transgenic plants in (A)