Fig. 2

Expression and subcellular localization analysis of SiBRI1 and its orthologues in different tissues in foxtail millet. (A), Leaves (L), stems (S), dark-grown seedlings (DGS), roots (R), non-flowering spikelets (NFS), and dry seeds (DS). Error bars indicate the mean ± standard deviation (SD). N = 3. Statistically significant differences are indicated by different lowercase letters (p < 0.05, one-way ANOVA with Tukey’s significant difference test). (B), Confocal images indicate the localization of SiBRI1-eGFP in the roots of 3-day-old dark-grown seedlings overexpressing SiBRI1 with a GFP tag at the C-terminus. Scale bar = 20 µm