Fig. 3

Validation of nine defense-related or sample-specific differentially expressed genes using qRT-PCR. For RNA-Seq, normalized counts of a gene in each sample (normalized counts ± SD) were measured in five biological replicates. For qRT-PCR, the relative gene expression in each sample (ΔCt ± SD) was measured in four biological replicates and three technical repeats with a ubiquitin gene (Os03g0234200) used as a reference gene. OsICS1 = isochorismate synthase 1, OsAOS2 = allene oxide synthase 2, OsLOX = lipoxygenase, OsPAL1 = phenylalanine ammonia-lyase 1, OsPAL2 = phenylalanine ammonia-lyase 2, OsCHS1 = chalcone synthase 1, AK102138 = Bowman-Birk serine protease inhibitor family protein, expressed, AB246888 = NADPH oxidoreductase 1, AK064067 = conserved hypothetical protein, CL = control_leaf, CS = control_sheath, LL = leaffolder_leaf, LS = leaffolder_sheath, BL = BPH_leaf, BS=BPH_sheath. The measurements were analyzed with two-way ANOVA and LSD post hoc test with different letters denoting significant differences (p < 0.05)