Fig. 6

RT-qPCR validation of the gene expression profiles observed in the RNA-Seq data. The transcript levels of PvENOD2 (a), PvGS (b), PvSAT1 (c), PvALLB (d), PvUA (e), and PvCYCB2;3 (f) were measured in control and PvRbohB-RNAi transgenic roots at 7 dpi with R. tropici. The log₂(fold change) of the corresponding genes from the RT-qPCR and RNA-Seq data are represented by beige and oranges bars, respectively (a–f). The RT-qPCR data were normalized to the expression of PvEF1α, and bars show the means ± SE of at least three independent biological replicates, with three technical repeats (n > 10). The asterisks represent significant differences between non-inoculated and inoculated samples, as revealed using an unpaired Student’s t-test (*P < 0.05; ***P < 0.001)