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Fig. 3 | BMC Plant Biology

Fig. 3

From: Reduced free asparagine in wheat grain resulting from a natural deletion of TaASN-B2: investigating and exploiting diversity in the asparagine synthetase gene family to improve wheat quality

Fig. 3

Presence/absence of TaASN-B2 in UK wheat varieties. a-d: Electrophoresis gels of PCR products from assays to distinguish the presence and absence of TaASN-B2 in a collection of UK wheat varieties grown across two years [11]. a. UK Flour Millers milling group 1 and 2 hard bread wheats (grown in 2012–2013). b. UK Flour Millers group 3 and 4 soft wheats (grown in 2012–2013). c. UK Flour Millers group 4 hard wheats (grown in 2012–2013). d. Remaining varieties grown only in 2011–2012. Varieties Cadenza and Claire were used as controls for TaASN-B2 presence and absence, respectively. The distinguishing PCR products are indicated with arrows. e. Diagram showing the frequency of the TaASN-B2 deletion in 63 UK wheat varieties, separated into UK Flour milling groups: G1 (breadmaking), G2 (breadmaking potential), G3 (soft/biscuit), G4 (feed/other)

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