Fig. 5

The phenotypes of seedling in cold treatment. 7 DAG seedlings were shifted to -6 °C for 2, 3 and 4 h and then returned to normal conditions (22 °C) for 36 h. a The phenotypes of WS2, brs1-1 and brs1-1D grown on 1/2 MS medium under normal conditions and with cold treatment for 2 h, 3 h and 4 h. Scale bar = 2 cm. The black dots on the plate represent the growth position of the seedlings before the cold treatment. b The root elongation in WS2 and brs1-1D after a severe cold treatment. The root elongation was measured at 36 h after cold shock. Data is from three biological replicates. The same results were repeated at least two times. The asterisks indicate a statistically significant difference (Student’s t-test, ** p < 0.01)