Fig. 2

Differences in gene sequences and BRS1 transcription in wild type and BRS1 mutants. a The sequence difference of BRS1 in WS2, brs1-1 and brs1-1D. The number above the exon represent the exon 1–9 from 5’ to 3’. A single thymidine base (red) is inserted in the first intron of brs1-1, and the 4 × 35S enhancer (four ellipses in black box) is inserted in the promoter of BRS1 in brs1-1D. b Transcription level (FPKM value) of BRS1 in WS2, brs1-1 and brs1-1D. All data were from RNA-seq and shown as mean ± SD. Three biological replicates were quantified. The asterisks indicate a statistically significant difference (Student’s t-test, *p < 0.05). c Alternative splicing event identification in brs1-1D. “isoform1” is the form in the TAIR 10 annotation. “isoform2” is newly identified in our result. d The relative percentages of isoform1 and isoform 2 in WS2 and brs1-1D. The asterisks indicate a statistically significant difference (**FDR-adjusted pvalue < 0.01)