Fig. 4

Fertility analysis of FLA14 knockout mutant (fla14) and overexpression (OE) transgenic Arabidopsis plants. a-c Morphological observation of wild-type (WT) (a), fla14 (b), and OE (c) plants. d-i A WT floret (d, g), a fla14 floret (e, h), and an OE floret (f, i). j-l Comparison of silique development in WT (j), fla14 (k), and OE (l) plants. The OE siliques were smaller than the normal siliques of fla14 and WT plants. m–o Ethanol-fixed siliques of WT (m), fla14 (n), and OE (o) plants. The OE siliques show few mature ovules. p Length of mature siliques in WT and OE plants. The values are the mean ± standard deviation (SD). Asterisks indicate significantly different means (p < 0.01) using one-way ANOVA. q Number of seeds per silique in WT and OE plants. The values are the mean ± standard SD. Asterisks indicate significantly different means (p < 0.01) using one-way ANOVA. Scale bars = 5 cm (a-c); 1 mm (d-i); 2 cm (j-l); 5 mm (m–o)