Fig. 3

Expression of FLA14 in Arabidopsis T-DNA-tagged mutant and overexpression (OE) transgenic plants. a Schematic representation of the insertion sites for CS1014037 and SALK_123695 mutants. The grayish box represents the coding region, the dark gray box indicates the 5’-untranslated region (5’-UTR) and the white box corresponds to the promoter region. The CS1014037 mutant contains a T-DNA insertion in the coding region of FLA14 699 bp downstream of the first ATG. The SALK_123695 mutant contains a T-DNA insertion located 945 bp upstream of the FLA14 mRNA initiation site. Three dark gray triangles indicate the position of three primer pairs used for quantitative real-time PCR analysis. b Map of the T-DNA portion of the binary vector used in the FLA14 OE construct. c, d Quantitative real-time PCR analysis of FLA14 in the homozygous CS1014037 line (fla14-1) and SALK_123695 line (fla14-2) (c) and the OE lines (d) compared with wild-type (WT) plants. The values are the mean ± standard deviation (SD). Asterisks indicate significantly different means (p < 0.05) using one-way ANOVA. RNA is extracted from whole inflorescences. Ubiquitously expressed Tubulin beta-4 was used as an internal control