Fig. 10

Calcofluor white staining of microspores from wild-type (WT) and FLA14-overexpressing (OE) transgenic Arabidopsis plants. a-d, f-i Microspores from WT plants. f-i The corresponding bright field images. e An anther at anthesis stage from OE plants. Asterisks indicate aberrant pollen grains with a significant increase in the calcofluor white fluorescent signals. j-s Microspores from OE plants. o-s The corresponding bright field images. l, m, q, r Arrows indicate the aborted pollen grains with no detectable calcofluor white fluorescence. n, s Comparison of aberrant pollen grains with unusually strong calcofluor white fluorescence (indicated by asterisks) and normal mature pollen grains from OE plants. a, f, j, o Early uninucleate stage. b, g, k, p Late uninucleate stage. c, h, l, q Bicellular stage. d, i, m, r Tricellular stage. e, n, s Anthesis stage. Scale bars = 20 μm (a-d, f-s); 50 μm (e)