Fig. 1

Analysis of FLA14 expression in Arabidopsis. a Quantitative real-time PCR analysis of FLA14. The values are the mean ± SD (standard deviation). b-f GUS staining in proFLA14::eGFP-GUS transgenic plants. b A stained inflorescence. Staining was restricted to anthers at floral stages 11–14 and stigmas and styles at floral stages 12–14 (indicated by arrows). c Magnified image of a floret at stage 13. The stained transmitting tract is indicated by an arrow. d Magnified image of an anther at stage 12. e Magnified image of an unstained petal at stage 12. f An unstained 9-day-old seedling. g, h GFP expression in proFLA14::eGFP-GUS pollen grains. g Stamens of proFLA14::eGFP-GUS flowers compared with wild-type flowers (h) at floral stage 12. R, root; Ste, stem; L, young leaf; F, flower; Si, germinal silique. Scale bars = 1 mm (b); 500 μm (c, e, f); 250 μm (d, g, h)