Table 4 Effect of second chilling stress in seedlings on ROS scavenging enzymes

		NA-Ye	CA-Ye	NA-MP	CA-MP	NA-DP	CA-DP
Total CAT U/gFW	BCS	0.242 ± 0.131	0.284 ± 0.217	0.702 ± 0.032	0.312 ± 0.127*	0.325 ± 0.0305	0.176 ± 0.04
	ACS	0.609 ± 0.276	0.684 ± 0.047	0.711 ± 0.109	222.428 ± 18.284**	0.53 ± 0.159	257.513 ± 20.042**
Total POD U/gFW	BCS	5.153 ± 0.876	6.275 ± 1.931	5.294 ± 0.529	6.907 ± 2.525	5.212 ± 2.235	7.102 ± 1.52
Total POD U/gFW	ACS	4.59 ± 0.141	5.997 ± 2.403	4.631 ± 1.399	5.66 ± 0.468	7.023 ± 1.209	7.638 ± 1.13
Total SOD U/gFW	BCS	4.816 ± 1.276	3.303 ± 1.089	5.685 ± 0.500	6.18 ± 1.352	4.233 ± 1.054	4.49 ± 0.582
	ACS	6.19 ± 1.065	5.878 ± 1.08	4.855 ± 1.123	6.07 ± 1.968	5.618 ± 1.296	7.865 ± 1.213

Catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) quantification in yellow- (Ye-), medium purple- (MP-), and deep purple- (DP-) seed groups of seedlings germinated under non-acclimated (NA) (18 °C) and chilling acclimated (CA) (4 °C) conditions. Data are means ± SD of three biological replicates. Significant differences, evaluated by t-test, are marked by asterisks: *p ≤ 0.05 or **p ≤ 0.01 when compared when compared to NA-BCS vs. CA-BCS and NA-ACS vs. CA-ACS within seed color group

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