Fig. 4

Heatmap showing the effect of the second chilling stress in gene expression. ROS (reactive oxygen species) scavenging genes; flavonoid, anthocyanins, and transcription factors regulating the flavonoid biosynthesis pathway; and cold response genes were measured in yellow- (Ye-), medium purple- (MP-), and deep purple- (DP-) seed groups of seedlings germinated under non-acclimated (NA) (18 °C) and chilling acclimated (CA) (4 °C) conditions before chilling stress (BCS) (18 °C) and after chilling stress (ACS) (4 °C for 6 h). Ye BCS and Ye ACS sample were used for comparison within groups (NA and CA). Numbers represent the fold changes. Relative color values are based of the subtraction of the row median, divided by row standard deviation. Data are presented as means ± SD of three biological replicates