Fig. 5

AtGLK1 participates in the plastid retrograde signal-mediated anthocyanin accumulation in Arabidopsis. a Real-time quantitative PCR analysis of AtGLK1 transcript accumulation in wild-type seedlings grown on 1/2 MS medium supplemented without (Control) or with 5 μM norflurazon (NF) or with 0.5 mM lincomycin (Linc). b Images of representative seedlings of the wild-type (Col), glk1 glk2 double mutant, and AtGLK1-overexpressing (OEGLK1-1 and OEGLK1-2) seedlings grown for 4 days on 1/2 MS medium supplemented without (Control) or with 5 μM norflurazon (NF) or with 0.5 mM lincomycin (Linc). c Absolute anthocyanin contents and relative anthocyanin contents d of the wild-type, glk1 glk2 double mutant, and AtGLK1-overexpressing seedlings grown on 1/2 MS medium for 4 days. The asterisks indicate statistically significant differences compared with the corresponding wild-type (Student’s t test: *P < 0.05)