Fig. 3

Anthocyanin accumulation in the wild-type and AtGLK1-overexpressing seedlings. a Expression analysis of AtGLK1 and AtGLK2 b in the wild-type (Col) and 35S::AtGLK1 transgenic lines (OEGLK1-1 and OEGLK1-2). Total RNA extracted from the 4-d-old wild-type and 35S::AtGLK1 transgenic seedlings was used for real-time quantitative PCR analyses. c Images of representative seedlings of the wild-type and 35S::AtGLK1 transgenic lines grown for 4 days on 1/2 MS medium supplemented without sucrose (-S), with 2% sucrose (+ S), or with 58 mM mannitol (Man), respectively. The black arrows indicate the locations of anthocyanin accumulation in different genotypic Arabidopsis seedlings. d Quantitative measurement of anthocyanins in the 4-day-old seedlings (Col, OEGLK1-1 and OEGLK1-2) grown on 1/2 MS medium supplemented without sucrose (-S), with 2% sucrose (+ S), or with 58 mM mannitol (Man), respectively. The asterisks indicate statistically significant differences compared with the corresponding wild-type (Student’s t test: *P < 0.05)