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Fig. 6 | BMC Plant Biology

Fig. 6

From: Two β-glucuronosyltransferases involved in the biosynthesis of type II arabinogalactans function in mucilage polysaccharide matrix organization in Arabidopsis thaliana

Fig. 6

Pectin deposition is altered in glcat14a-1 single mutants and glcat14a-1 glcat14c-1 double mutants. Immunolabelling with CCRC-M35 (a, d), JIM5 (b, e) and JIM7 (c) of the adherent mucilage and counterstained with S4B. Biochemical determination of calcium content and degree of pectin methylesterification expressed as a percentage (f). Uronic acid estimation (g) was determined using the meta-hydroxydiphenyl method (see Methods). Percentage (%) increase or decrease relative to WT is indicated in F. Values represents the means ± SD of four biological replicates. The single asterisk indicates significant differences compared to WT (Student’s t-test, P < 0.05 for single asterisks). Bars = 100 μm; DM-degree of methylesterification

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BMC Plant Biology

ISSN: 1471-2229

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