Table 2 Candidate QTL for Fe concentration and bioavailability identified through interval (IM) and composite interval mapping (CIM) using mpMap [35]. For each QTL, the table shows the mapped chromosome (Chr) and location (Pos), parental effects with the founder Xi19 used as a baseline, the flanking array markers, the Wald test statistic (Wald) and associated P value significance thresholds. The P values expressed to –log10 and the percentage phenotypic variation explained by each QTL (% Var) are also included. The results shown were extracted after fitting a multiple QTL model implemented through the mpMap function ‘fit()’
Year | Method | Flanking Markers (left – right) | Chr | Pos (cM) | Al | Br | Cl | He | Ri | Ro | So | Xi | Wald | -log10 | % Var |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Fe concentration | |||||||||||||||
1 | IM | RAC875_c67311_429 – RFL_Contig4718_1323 | 2B | 216.09 | -15.7 | -33.9 | -7.0 | -31.8 | -14.5 | -19.6 | -19.3 | 0 | 28.08*** | 3.7 | 7.7 |
1 | CIM | Kukri_c148_1484 – BobWhite_c1149_539 | 2B | 29.5 | -0.2 | -1.9 | -1.1 | -0.1 | -2.6 | -2.0 | -2.8 | 0 | 20.35** | 2.3 | 4.8 |
1 | CIM | RFL_Contig4718_1323 – BS00092235_51 | 2B | 217.5 | -3.1 | -9.8 | -1.6 | -19.5 | 3.3 | -8.2 | -8.1 | 0 | 43.72*** | 6.6 | 7.4 |
1 | IM | BS00039852_51 – RAC875_c8313_72 | 3D | 46.84 | 5.0 | 0.6 | -1.5 | 3.9 | -8.9 | -2.8 | -2.5 | 0 | 18.75** | 2.1 | 7.7 |
1 | CIM | BobWhite_c42020_456 – Ex_c4296_1270 | 3D | 180.63 | -3.2 | 3.3 | 1.4 | -1.5 | 2.8 | 0.2 | 3.4 | 0 | 23.62** | 2.9 | 3.9 |
1 | IM | tplb0023j07_1091 – RAC875_c63933_184 | 5D | 175.5 | 0.0 | -0.5 | -1.8 | 1.4 | -2.0 | -1.8 | 0.8 | 0 | 16.66* | 1.7 | 7.7 |
1 | CIM | BS00055493_51 – D_GB5Y7FA02JRQ1I_101 | 5D | 199.08 | -1.3 | -0.8 | -6.5 | -0.6 | -6.7 | -2.8 | -1.7 | 0 | 37.9*** | 5.5 | 6.5 |
1 | IM | TA004558_1018 – Ra_c14408_576 | 6 A | 128.93 | -3.2 | -2.0 | -1.1 | -2.0 | -4.0 | -1.4 | -0.7 | 0 | 22.86** | 2.7 | 8.0 |
1 | CIM | TA004558_1018 – Ra_c14408_576 | 6 A | 128.93 | -4.5 | -3.1 | -2.0 | -2.4 | -5.0 | -2.2 | -1.8 | 0 | 38.43*** | 5.6 | 8.0 |
2 | IM | BS00012942_51 – Tdurum_contig42013_538 | 2 A | 252.8 | -0.4 | 0.3 | -2.9 | -2.1 | -1.2 | -4.0 | -4.1 | 0 | 26.08*** | 3.3 | 6.3 |
2 | CIM | BS00012942_51 – Tdurum_contig42013_538 | 2 A | 252.8 | -0.6 | 0.2 | -2.9 | -2.2 | -1.3 | -4.0 | -4.2 | 0 | 26.01*** | 3.3 | 6.3 |
Fe bioavailability | |||||||||||||||
1 | IM | TA005289_1104 – IAAV3156 | 1 A | 167.31 | -0.2 | -0.8 | 0.5 | 0.5 | -0.5 | 1.2 | 0.8 | 0 | 16.61* | 1.7 | 8.1 |
1 | IM | BS00079088_51 – BS00065268_51 | 1 A | 193.67 | -0.4 | -2.1 | 1.6 | -1.3 | -0.1 | -0.9 | -0.6 | 0 | 17.38* | 1.8 | 8.4 |
1 | CIM | BS00065268_51 – Kukri_c310_1953 | 1 A | 195 | -0.6 | -2.8 | 2.6 | -1.1 | -0.6 | -0.4 | -0.2 | 0 | 36.65*** | 5.3 | 8.2 |
1 | CIM | wsnp_Ex_c35331_43499339 – wsnp_JD_rep_c48914_33168544 | 2 A | 87.5 | -1.3 | 0.3 | -0.7 | -1.1 | -2.3 | -0.5 | -0.6 | 0 | 28.89*** | 3.8 | 5.8 |
1 | CIM | BS00022498_51– RAC875_c1638_165 | 7B | 72.41 | 0.3 | -1.2 | -1.7 | -0.1 | 0.0 | 0.3 | -0.1 | 0 | 25.29*** | 3.2 | 3.6 |
2 | CIM | Excalibur_c12980_2392 – wsnp_Ra_c8771_14786376 | 2 A | 10.5 | -0.4 | 3.4 | 1.8 | -0.9 | 2.5 | 2.6 | 1.7 | 0 | 24.6*** | 3.0 | 4.5 |
2 | CIM | BS00084904_51– Excalibur_c100336_106 | 4B | 55.7 | -0.5 | -2.2 | -0.6 | -1.4 | 0.4 | -3.0 | 1.4 | 0 | 24.2** | 3.0 | 4.4 |
Al Alchemy, Br Brompton, Cl Claire, He Hereward, Ri Rialto, Ro Robigus, So Soissons, Xi Xi19 | |||||||||||||||
*** = <0.001; ** = <0.01; * = <0.05. | |||||||||||||||