Fig. 2

Multiple sequence alignment of CETS proteins. a Amino acid alignment of the CETS proteins from Physcomiterella patens (Pp), Arabidopsis thaliana (At), Solanum lycoperscium (Sl), Gossypium hirsutum (Gh), and Theobroma cacao (Tc) is displayed. The red asterisk indicates the important His-88/Tyr-85 residue critical for determining floral activating or repressive activity. The black asterisks mark residues shown to interact with 14–3-3 proteins. Red boxes highlight the conserved DPDxP, GxHR and L/IYN motifs, respectively. A black box marks the external loop portion of the ligand binding domain. Segments A-D of exon 4 as defined in (34) are underlined and labeled. Protein, species, and accession numbers for aligned sequences are listed in Supplemental Table 3 (Table S3). b DNA coding sequence (cds) alignment of T. cacao Criollo FT (Tc05v2_g009810, reference genome) and Scavina6 FT (study genotype). Scavina6 FT coding sequence is a consensus of alignment of cloning sequencing results (4 clones) to Criollo FT. Clone sequences had 100% identity to both the consensus and (as pictured) Criollo’s FT coding sequencing (data not shown)