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Table 3 GFP and VIGS insert stability in constructs pWX27, pWX68, and pWX56

From: Simultaneous gene expression and multi-gene silencing in Zea mays using maize dwarf mosaic virus

Construct Insert Rep.a Time (dpi) Total plants ab b c d e
pWX27 GFP 1 7 20 9 10 0 0 1
2 7 20 6 13 1 0 0
3 7 20 11 8 0 1 0
1 14 20 0 18 2 0 0
2 14 20 0 13 4 2 1
3 14 20 0 16 4 0 0
1 21 20 0 5 12 2 1
2 21 20 0 3 9 8 0
3 21 20 0 3 12 5 0
pWX68 GFP 1 7 20 14 4 2 0 0
2 7 20 2 11 4 3 0
3 7 20 3 10 5 2 0
1 14 20 10 6 2 2 0
2 14 20 3 6 5 5 1
3 14 20 2 4 6 8 0
1 21 20 3 9 4 3 1
2 21 20 0 0 12 7 1
3 21 20 0 2 7 9 2
pWX56 GFP 1 7 20 7 6 1 4 2
2 7 20 11 4 0 3 2
3 7 20 8 5 1 1 5
1 14 20 9 7 2 2 0
2 14 20 3 14 1 2 0
3 14 20 8 7 3 0 2
1 21 20 2 8 2 4 4
2 21 20 5 10 0 4 1
3 21 20 0 12 2 5 1
pWX56 VIGS 1 7 20 6 9 5 0 0
2 7 20 17 0 0 3 0
3 7 20 0 16 0 0 4
1 14 20 13 2 3 0 2
2 14 20 6 12 0 1 1
3 14 20 11 4 0 5 0
1 21 20 12 3 0 1 4
2 21 20 12 3 0 1 4
3 21 20 4 4 1 1 10c
  1. aThree experimental replicates (20 plants per construct in each), were tested by RT-PCR. Wild type pWX6 and healthy plants were used as positive and negative controls
  2. bRT-PCR results per sample were scored into 5 different categories (a,b,c,d and e), based on band(s) observed: a full length insertion single band observed, b multiple bands observed; including expected full-length insertion and smaller bands indicating insert loss, c multiple bands indicating insert loss, with none representing full length insertion; d single band detected at less than full-length insertion size, usually close to size of wild type virus amplicon; e no bands detected with primer pair
  3. cThis replicate was excluded from statistical analyses since no amplification was observed in half of the samples