Fig. 7

Silencing of BrFLU in Y-05 is critical for ALA biosynthesis. a The phenotype of pTY plants (CK) and pTY-FLU (BrFLU-silenced) plants. Bar = 5 cm. b The relative expression level of BrFLU significantly decreased in pTY-FLU plants. The inner leaves were selected to confirm the BrFLU expression. c The pTY-FLU plants showed higher ALA content than pTY plants. d The pTY-FLU plants showed higher chlorophyll content than pTY plants. e The phenotype of pTY and pTY-FLU plants after cold acclimation. Bar = 1 cm or 2 mm. f The pTY-FLU plants showed higher chlorophyll content than pTY plants after cold acclimation. Chlorophyll a, Chlorophyll b and total chlorophyll abbreviated as Chl a, Chl b, Total Chl, respectively. Three individual plants of each cultivar were quantified, and the chlorophyll content was measured three times. Error bars represent SE (±SE, n = 3). * represents p < 0.05, ** represents p < 0.01