Table 1 Summary of small RNA sequencing results
Sample | S1 | S2 | S3 | S4 |
|---|---|---|---|---|
Total reads | 19,572,690 (100%) | 14,258,144 (100%) | 13,540,799 (100%) | 15,438,497 (100%) |
N% > 10% | 491 (0.00%) | 223 (0.00%) | 22 (0.00%) | 41 (0.00%) |
Low quality | 8922 (0.05%) | 5375 (0.04%) | 3203 (0.02%) | 4338 (0.03%) |
5′ adapter contamination | 24,207 (0.12%) | 18,667 (0.13%) | 13,689 (0.10%) | 14,148 (0.09%) |
3′ adapter null or insert null | 943,999 (4.82%) | 740,543 (5.19%) | 263,635 (1.95%) | 320,439 (2.08%) |
With ploy A/T/G/C | 41,579 (0.21%) | 10,186 (0.07%) | 38,713 (0.29%) | 10,100 (0.07%) |
Clean reads | 18,553,492 (94.79%) | 13,483,150 (94.56%) | 13,221,537 (97.64%) | 15,089,431 (97.74%) |
Total sRNA | 10,436,878 (100%) | 8,007,808 (100%) | 10,348,535 (100%) | 11,058,825 (100%) |
Mapped sRNA | 8,109,791 (77.70%) | 7,269,789 (90.78%) | 6,456,441 (62.39%) | 10,301,776 (93.15%) |
“+” Mapped sRNA | 7,381,437 (70.72%) | 6,504,225 (81.22%) | 5,689,563 (54.98%) | 9,825,133 (88.84%) |
“-” Mapped sRNA | 728,354 (6.98%) | 765,564 (9.56%) | 766,878 (7.41%) | 476,643 (4.31%) |