Fig. 6

Phenotypical analyses of silencing of five GhGRF genes in G. hirsutum. Phenotypes of TRV:00 and TRV:GhGRF3 (a), TRV:GhGRF6 (b), TRV:GhGRF9 (c), TRV:GhGRF15 (d), and TRV:GhGRF14 (e). Relative expression of GhGRF3 (f), GhGRF6 (g), GhGRF9 (h), GhGRF15 (i), and GhGRF14 (j) in TRV:00 and TRV:GhGRFs plants. Ubiquitin7 (GhUBQ7, GenBank accession no. DQ116441) gene was used as an internal reference transcript. Values are means ± SD (n = 3). Asterisks indicate significant differences between control TRV:00 and TRV:GhGRFs silencing lines (Student’s t test, **P < 0.01, ***P < 0.001, ****P < 0.0001). Scale bar, 30 cm