Fig. 6

Functional identification of GhlncLOX3 against V. dahliae using virus-induced gene silencing (VIGS). a GhlncLOX3 gene expression fold change and disease index of infected plants in eight cultivars. ‘MT’ and ‘VD’ mean mock treatment and seedling roots inoculated with V. dahliae, respectively. b Predicted base-pairing interactions between GhlncLOX3 and GhLOX3. c Phenotypes of seedlings with GhlncLOX3 silencing post inoculation, showing the wilting and etiolated phenotype. Cloroplastos alterados 1 (CLA1) was used as the positive gene. d Disease index of infected plants. e The qRT-PCR verification of GhlncLOX3 silenced by VIGS. f Expression change level of GhLOX3 after silencing GhlncLOX3. g Content change level of JA after silencing GhlncLOX3. h The correlation between GhlncLOX3 (X-axis) and GhLOX3 (Y-axis) expression levels in 14 samples was detected by Pearson correlation analysis. Red dots represent GhlncLOX3-silenced plants non-inoculated with V. dahliae. Blue dots represent GhlncLOX3-silenced plants inoculated with V. dahliae. i A schematic representation of speculative processes involved in GhlncLOX3 raising the resistance of cotton to Verticillium dahliae. 13(S)-HPOT represents 13S-hydroperoxy-6Z, 9Z, 11E-octadecatrienoic acid, an intermediate product of JA synthesis. ** indicated significantly different (Student’s t-test, P < 0.01) in d, e, f, g