Fig. 6

Drought tolerance analysis of the Gh4CL7-overexpressing Arabidopsis plants. a The relative expression level of Gh4CL7 in three independent Gh4CL7-OE Arabidopsis plants. b Germination rate of the Gh4CL7-OE Arabidopsis seeds on 1/2 MS supplemented with 0 and 300 mM mannitol. c-d Root elongation of the 6-days-old Gh4CL7-OE Arabidopsis seedlings on 1/2 MS supplemented with 0, 200, and 300 mM mannitol. Bar = 1 cm. e Phenotypic assay of the Gh4CL7-OE and WT Arabidopsis plants during drought treatment. f-h The contents of H₂O₂ and MDA and antioxidative enzyme activity in Gh4CL7-OE and WT plants under normal and water deficit conditions. Ten-days-old seedlings were transplanted to soil and regularly watered for 2 weeks. For drought treatment, irrigation was terminated for 2 weeks. For the rehydration treatment, the plants were re-watered 3 days after the drought treatment