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Table 4 Differentially expressed novel miRNAs associated with grain development and their target gene functions

From: Identification of microRNAs in developing wheat grain that are potentially involved in regulating grain characteristics and the response to nitrogen levels

Grian filling stagesmiRNAAbundance (RPM)P- valueAbundance (RPM)P- valueTarget function
LN-7LN-17LN-27HN-7HN-17HN-27
Relative abundance during grain filling stages (RAGS)tae-miR2916-p5_2ss2AG17TG1070366795572.49E-061610206728681.52E-05protein serine/threonine kinase; glutathione S-transferase (GSTF)1; DELLA
tae-miR2916-p3_2ss2TC17CA2518711027,7365.92E-083507461070491.66E-06Peroxidase; fructose-bisphosphate aldolase; GTPase
tae-miR9655-p5293624,65711,3292.48E-0566820,12011,5541.30E-09transcription factor; oxidation-reduction
Relative abundance at early stages(RAES)tae-miR166d-p310746705.58E-038356801.97E-02cell differentiation; kinesin-1-like protein PSS1
PC-5p-30718_16439706.33E-05492154.94E-05BRASSINOSTEROID INSENSITIVE 1; 1,3-beta-D-glucan synthase
tae-miR815c-p5_2ss6CT21CT10792213.62E-0412161514.76E-04UDP-arabinose 4-epimerase 1-like; transcription factor TGA2-like; Dnaj
tae-miR9662b-p512834155.04E-0715331184.56E-05beta-fructofuranosidase
tae-miR9662a-p59562465.53E-0411277673.96E-02beta-fructofuranosidase;
tae-miR9672b-p51253301.57E-049430101.65E-03LRR receptor-like serine/threonine-protein kinase FLS2-like
tae-miR9779-p324440111.15E-0336143939.91E-04hexose transmembrane transport
Relative abundance at middle stages (RAMS)tae-miR5384-p55776271327.30E-065796864891.07E-03UDP-glycosyltransferase 83A1-like; ethylene-responsive transcription factor 1B
PC-5p-3645_2157331751481.88E-05411541051.81E-05Metalloendopeptidase; SCF ubiquitin ligase
PC-3p-4780_175042311013.90E-0501351041.09E-06aspartic endopeptidase; transcription factor
PC-5p-24289_24722331.20E-03026166.58E-04NEDD8-specific protease; protein ECERIFERUM 1-like
PC-5p-18073_397066263.34E-03069263.51E-06beta-glucosidase; BRASSINOSTEROID INSENSITIVE 1-like
PC-3p-21502_303233401.30E-033830251.03E-02Metalloendopeptidase; aspartic-type endopeptidase;MYB
tae-miR164-p3681591431.24E-03571651313.76E-03auxin response factor 3-like; transcription factor IIB
tae-miR164b-p31662181.71E-031640213.79E-03auxin response factor 2-like; high-affinity nitrate transporter 2.1-like
tae-miR1137a-p5_2ss9GA20GC02823.31E-0201321.77E-03DNA mismatch repair protein MSH6
tae-miR2275-p5026131.82E-0402821.66E-04LRR receptor-like serine/threonine-protein kinase FLS2; BRASSINOSTEROID INSENSITIVE 1
PC-5p-21100_312035172.14E-0302792.75E-03glycerol-3-phosphate dehydrogenase; protein gar2
PC-3p-15070_514061257.94E-04044412.64E-06transcription factor
tae-miR9653b-p363658.36E-03202105.81E-04protein binding
PC-3p-11233_746386596.74E-052258331.38E-02Metalloendopeptidase
PC-3p-16735_4451043133.99E-06038231.72E-05O-acyltransferase WSD1-like
PC-5p-12716_6420101751.14E-040111796.00E-04MAPK cascade; BRASSINOSTEROID INSENSITIVE 1
tae-miR6300-p5233122684.86E-05252582531.42E-05protein RPM1; response to nitrate
Relative abundance at later stages (RALS)tae-miR2592bj-p3_2ss12TC19AT821933806.17E-041142171354.57E-04protein serine/threonine phosphatase; transporter; protein RPM1-like
PC-5p-31845_15408214.35E-0308115.36E-03receptor-like serine/threonine-protein kinase SD1–6
PC-5p-1231_4743522603721.48E-06403394531.35E-07serine-type endopeptidase
PC-5p-13909_573047666.93E-05042567.87E-05GTPase
PC-3p-13925_5731711551.16E-0659876.72E-06serine-type carboxypeptidase; valine-tRNA ligase
tae-miR408-p5_1ss20GA16175.81E-0416229.12E-03BRASSINOSTEROID INSENSITIVE 1
  1. aAbundance is represented by normalized reads (reads per million of total miRNA reads (RPM))
  2. bRPM ≥20 in these libraries
  3. cThe values are the means of three biological replicates
  4. dP-value < 0.05 (5.00E-02) means a significant difference among different developmental stages