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Fig. 4 | BMC Plant Biology

Fig. 4

From: Nerylneryl diphosphate is the precursor of serrulatane, viscidane and cembrane-type diterpenoids in Eremophila species

Fig. 4

a Phylogenetic analysis of Eremophila CPTs. Maximum likelihood tree of CPTs based on aligned protein sequences calculated using MEGA 7 [56]. Tree is drawn to scale, with branch lengths representing the number of substitutions per site. Filled circles on branches indicate bootstrap support above 75% based on 1000 repetitions. Genbank accession numbers are listed in Tables S3 and S9 (Additional file 1). Eremophila CPTs in blue box were functionally characterised. NP = no main product detected. Subcellular localisation predicted using DeepLoc-1.0 [57]. b In vivo functional characterisation of Eremophila CPTs. GC-MS chromatograms of hexane extracts of E. coli cultures expressing Eremophila CPTs in combination with ElTPS31

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