Fig. 2

Metabolomic analyses and changes in the expression of photosynthesis-related genes of A. thaliana under different elevated temperature treatments. a Metabolome view map of the common metabolites identified in the plants subjected to prolonged warming and heat shock; the different colour depth of circles represent the p-value of pathway enrichment analysis. b List of metabolites significantly affected by heat shock (HS) compared to control (CK), prolonged warming (PW) compared to control (CK), prolonged warming (PW) compared to heat shock (HS) (p-value ≤0.05), organized by functional categories and corresponding accumulation fold-changes. The numbers represent fold-changes. Red shading means up-regulation and green shading means down-regulation. c Pathway diagram of light and dark reactions of photosynthesis with superimposed color-coded squares showing DEGs. Red squares: up-regulated genes; blue squares: down-regulated genes. Left-hand column: heat shock vs control; middle column: prolonged warming vs control; right-hand column: prolonged warming vs heat shock. CK: control; PW: prolonged warming; HS: heat shock. LHC I/II: light-harvesting complex I/II; PS I/II: photosystem I/II; PQ: plastoquinone; Cyt: cytochrome b6f complex; PC: plastocyanin; Chl: chlorophyll; Fdx: ferredoxin