Fig. 5

Concentrations of fructose (a) and glucose (b), and transcript abundance of their metabolism-related genes (c-f) in apple fruit tissues in response to fruit load reduction. Treatment was performed at 11 d after full bloom. Transcript abundance was measured using quantitative RT-PCR analysis. Mean and S.D. of metabolite concentration data are presented (n = 4). The mean and S.E of the mean (n = 4) of transcript abundance are displayed. CC: Control fruit load-Cortex; CP: Control fruit load-Pith; RC: Reduced fruit load-Cortex; RP: Reduced fruit load-Pith. FK: FRUCTOKINASE; HXK: HEXOKINASE. Transcript abundance is in reference to mean expression at 0 d after treatment in RC. These data were normalized to that of ACTIN and GAPDH (GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE). Asterisk and dagger symbols indicate significant difference between control and reduced fruit load treatments in the cortex and pith, respectively (α = 0.05). Shaded regions in the background indicate early (dark grey), mid (light grey), and late (white) fruit development periods