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Fig. 7 | BMC Plant Biology

Fig. 7

From: Characterization of wheat homeodomain-leucine zipper family genes and functional analysis of TaHDZ5-6A in drought tolerance in transgenic Arabidopsis

Fig. 7

Phenotype of the 35S:TaHDZ5-6A transgenic Arabidopsis. a RT-PCR analysis of TaHDZ5-6A transcript levels in the three transgenic lines. b Statistical analysis of survival rates after the drought-stress treatment. The average survival rates and standard errors were calculated based on data obtained from three independent experiments. Significant differences were determined by a t-test. *P < 0.05, **P < 0.01. c Drought tolerance of 35S:TaHDZ5-6A transgenic Arabidopsis. Photographs were taken before and after the drought treatment, and followed by a six-day period of re-watering. D Stomatal aperture of WT and 35S::TaHDZ5-6A transgenic plants under normal and drought conditions. e Statistical analysis of stomatal aperture of WT and 35S::TaHDZ5-6A transgenic plants. Values are mean ratios of width to length. Error bars represent standard errors of three independent experiments (n = 60). Bars, 10 μm. f Water loss from detached rosettes of WT and 35S::TaHDZ5-6A transgenic plants. Water loss was expressed as the percentage of initial fresh weight. Values are means from eight plants for each of three independent experiments. Significant differences were determined by a t-test. *P < 0.05, **P < 0.01. g Free proline content of WT and 35S::TaHDZ5-6A transgenic plants under normal and drought stress treatment

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