Fig. 7

Homo-oligomerization of TaSTP13. a TaSTP13 can form homodimers within itself detected by the split-ubiquitin system using LacZ as a reporter gene. APP (amyloid A4 precursor protein) and Fe65 (amyloid beta A4 precursor protein-binding family B member 1) were used as positive control. Interactions of a TaSTP13-Cub fusion with a WT variant of NubI (internal positive control) or mutant variant of NubG (internal negative control) and TaSTP13-Nub fusion were tested. Cells of yeast strain NMY51 harboring the indicated plasmid combinations were grown on selective media (containing X–gal). Positive interaction was visualized by β-galactosidase expression in drop assays. Yeast growth assays on an SD medium (− Leu, − Trp, − Ade, and - His). b BiFC assays for TaSTP13 homooligomerization. Yn:TaSGT1 + TaRAR1:Yc (positive control) is shown in the top three panels, Yn:TaSTP13 + TaSTP13:Yc is shown in the middle three panels, and Yn:TaSTP13 + Yc (negative control) is shown in the lower three panels. Agrobacterium-mediated transient expression of indicated constructs in N. benthamiana leaves. Bright field and YFP fluorescence (in green) images were taken by confocal microscopy and merged. All assays were repeated independently at least two times with comparable results