Fig. 5

– Showing shoot formation pattern: a initial fresh tissue that was used as a cross-sectioned stem cultured with the upper end up (CUEU) or cross-sectioned stem cultured in inverted position (IC) explant to induce multiple shoots; b a CUEU explant with multiple shoot buds and shoots growing at the explant base inserted into the medium; c an IC explant with multiple shoot buds and shoots growing at the aerial base of inverted explant; d initial fresh tissue that was used as LWS explant, for culture; e a growing length-sectioned stem (LWS) explant with a more expanded, twisted wound surface area with multiple shoot buds and shoots along length-sectioned wound; f sample clusters of multiple shoot buds and shoots subdivided from a growing CUEU b, IC c or LWS e explant, for continuous induction of repeated multiple buds and shoots; g repeated multiple shoot buds and shoots resulting from subdivisions of multiple buds and shoots clusters in f of a single LWS explant