Fig. 3

Expression pattern of NtPSY genes. a Expression levels of NtPSY genes in leaves, stems, flowers, and roots; the relative expression levels in each tissue were calculated by setting the expression value of NtPSY2–1 + NtPSY2–2 in roots as 1. b Changes in the expression of NtPSY genes under treatment with different phytohormones; the relative expression levels under different conditions were calculated by setting the expression value of NtPSY2–1 + NtPSY2–2 in the control as 1. c Changes in the expression of NtPSY genes under strong light conditions; the relative expression levels at different intervals were calculated by setting the expression value of NtPSY2–1 + NtPSY2–2 at 0 h as 1. Three pairs of conserved qPCR primers that can be used to estimate the sum expression of NtPSY1–1 and NtPSY1–2, NtPSY2–1 and NtPSY2–2, NtPSY3–1 and NtPSY3–2, respectively, were designed. Columns and bars represent the means and standard errors (n = 3), respectively. Columns marked by different letters indicate statistical significance (P < 0.05). The data for the expression of NtPSY3–1 and NtPSY3–2 are not shown as no expression was detected under all the conditions