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Fig. 3 | BMC Plant Biology

Fig. 3

From: Characterization of type-2 diacylglycerol acyltransferases in Haematococcus lacustris reveals their functions and engineering potential in triacylglycerol biosynthesis

Fig. 3

Functional characterization of HpDGAT2s in Saccharomyces cerevisiae cells. a TLC analysis of total lipids extracted from the control S. cerevisiae (INVSc1), TAG-deficient S. cerevisiae (H1246), and H1246 cells transformed with HpDGAT2s and Empty vector (EV). b and c The transcriptional expression levels of HpDGAT2s in H1246 and INVSc1 cells transformed with HpDGAT2s. The gene expression levels were normalized to the endogenous ACT1 gene. d TAG contents and relative abundance of fatty acid in INVSc1 and H1246 cells transformed with HpDGAT2s. e Relative abundance of fatty acid in H1246 cells transformed with HpDGAT2s by feeding of free fatty acid of C18:2n6, C18:3n3, C18:3n6, and C18:4n3 after a 24 h cultivation

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