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Table 1 AmpliSeq results and classification of markers

From: Targeted amplicon sequencing + next-generation sequencing–based bulked segregant analysis identified genetic loci associated with preharvest sprouting tolerance in common buckwheat (Fagopyrum esculentum)

 

Cross A_1 (n = 94)

Cross B_1 (n = 87)

Cross C (n = 84)

Sh linka

PHS linka

Whole-genomea

Total

Sh link

PHS link

Whole-genome

Total

Sh link

PHS link

Whole-genome

Total

Number of SNP detected markers

67

56

176

299

74

41

124

239

68

34

140

242

After filtering and before removing duplicateb

57

48

112

217

50

14

61

125

43

11

55

109

Remove duplicated markers (no. of duplicated marker)

12 (45)

18 (30)

98 (14)

127 (90)

10 (40)

10 (4)

54 (7)

74 (51)

5 (38)

8 (3)

49 (6)

62 (47)

 LG1

12 (45)

2 (1)

13 (2)

27 (48)

10 (40)

1 (1)

8 (3)

19 (44)

5 (38)

2 (1)

6 (3)

13 (42)

 LG2

0

0

11 (3)

11 (3)

0

0

10

10

0

0

4 (1)

4 (1)

 LG3

0

2 (3)

11 (4)

13 (7)

0

2 (2)

6

8 (2)

0

2 (1)

11

13 (1)

 LG4

0

7 (1)

11 (3)

18 (4)

0

4 (1)

7 (1)

11 (2)

0

4 (1)

6

10 (1)

 LG5

0

0

15

15

0

0

7

7

0

0

9

9

 LG6

0

7 (25)

11 (1)

18 (26)

0

3

1

4

0

0

3 (1)

3 (1)

 LG7

0

0

20

20

0

0

8 (1)

8 (1)

0

0

5

5

 LG8

0

0

5 (1)

5 (1)

0

0

7 (2)

7 (2)

0

0

5 (1)

5 (1)

  1. aExpected marker linkage: Sh-link, self-compatibility; PHS link, tolerance to preharvest sprouting; whole-genome, whole genome region
  2. bFiltering steps: drop duplicate markers; drop samples with < 80 genotypes; drop marker subsets in which < 90% of population is genotyped; drop markers with an abnormal genotyping distribution