Fig. 4
Expression pattern of VvNPF6.5. a, Tissue-specific expression of VvNPF6.5. qRT-PCR analysis was used to determine the relative expression level of VvNPF6.5 in various tissues and organs. L, leaf blades; P, petioles; S, stems; T, tendrils; R, roots; F, flowers; PE, peel; FL, flesh. Relative expression of VvNPF6.5 was normalized to that of VvACT1. b, Time-course analysis of VvNPF6.5 expression following nitrate induction. ‘Kyoho’ seedlings were grown in soil for 2 months and then grown hydroponically in nitrogen-starvation medium for 1 week. The plants were subjected to 6.4 mM NO3− or 0 mM NO3− for the indicated time. Total RNA was isolated from root tips and VvACT1 was used as the internal control. The relative level is the expression normalized to that of 0 h. Values are means ± SD, n = 3