Table 1 Major features of the de novo assembly of gerbera leaf transcriptome. Gerbera transcriptome was created from short read cDNA sequences of a powdery mildew-resistant (UFGE 4033) line and a susceptible (06–245-03) breeding line using four assemblers (Trinity, TransAbyss, SoapDenovo and Velvet pipelines)
Features | Values |
|---|---|
Transcript distribution | |
 number of transcripts (no.) | 145,348 |
 smallest transcript (bases) | 125 |
 largest transcript (bases) | 32,039 |
 Total number of nucleotides | 111,409,237 |
 mean transcript length (bases) | 761 |
 transcripts under 200 bases | 4903 |
 transcripts over 1000 bases | 31,896 |
 transcripts over 10,000 bases | 10 |
 Transcripts with open reading frames (ORF) | 67,312 |
 mean ORF percent | 81.21 |
Assembly length | |
 n90 | 358 |
 n70 | 617 |
 n50 | 1124 |
 n30 | 1837 |