Fig. 4

AtAHL20 overexpression represses FT expression under LD conditions. a FT transcript levels were suppressed to ~ 30% of wild-type levels in 35S:AtAHL20-1 and 35S:AtAHL20-2 plants. In contrast, FT transcript abundance was elevated in the dominant-negative mutant plants 35S:AtAHL20m1 and 35S:AtAHL20m2, but were unchanged in ahl20-1 and ahl20-2 T-DNA insertion lines. b RT-qPCR analysis data showed that AGL8, SPL3 and TSF transcript levels were repressed in 35S:AtAHL20 plants but were unchanged in the wild type and 35S:AtAHL20m1, 35S:AtAHL20m2, ahl20-1 and ahl20-2 plants. RT-qPCR analysis was performed using RNA extracted from rosette leaves of three biological replicates of 21-day old plants grown under LD conditions. The error bar denotes standard error of mean (SEM). Different letters indicate statistical significance (ANOVA; P < 0.05)