Fig. 8

Effect of Glyma.04G190700 RNAi on RNS in soybean. a Mean numbers of nodules per plant with altered Glyma.04G190700 transcript levels. The numbers of independent transgenic plants in each sample are indicated in parentheses. b Nitrogenase activities of nodules in control and Glyma.04G190700 RNAi plants. The vector p5941-35S-intron-(GFP-Bar marker) transgenic hairy roots were served as control (a and b). c qPCR analysis of the transcript levels of Glyma.04G190700, ENOD40, Nodulin35, Calmodulin and Lb1 in the control and transgenic hairy roots. d qPCR analysis of the transcript levels of Glyma.04G190700, ENOD40, Nodulin35, Calmodulin and Lb1 in the control and transgenic nodules. Total mixed RNA isolated from root system (including small nodules) or nodules of multiple independent transgenic plants was used for qPCR analysis. The references in the control hairy roots and nodules were used to calculate relative expression levels of these five genes in transgenic hairy roots and nodules. The data are presented as the mean ± SD and “ ” indicates statistical difference between different inoculated hair roots (t test, p < 0.01)