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Table 2 Frequency of Cas9-positive and Cas9-free T2 seeds

From: Simultaneous induction of mutant alleles of two allergenic genes in soybean by using site-directed mutagenesis

T1 plant numbera Cas9
integration
Number of T2 seeds
Mutation No mutation Total
Gly m Bd 28 K and Gly m Bd 30 K Gly m Bd 28 K only Gly m Bd 30 K only
E1–1 positive 16 10 3 0 29
free 2 1 5 2 10
E1–2 positive 21 0 0 0 21
free 1 0 3 0 4
E1–5 positive 8 4 0 1 13
free 1 0 3 0 4
E2–1 positive 0 20 0 5 25
free 0 2 0 5 7
E2–2 positive 1 20 0 0 21
free 0 0 0 5 5
K1–1 positive 9 1 0 0 10
free 3 0 0 0 3
K1–3 positive 13 3 0 0 16
free 3 0 4 2 9
K2–1 positive 16 3 1 0 20
free 1 0 2 0 3
K2–2 positive 19 7 0 0 26
free 1 0 4 0 5
K4–1 positive 33 0 2 0 35
free 2 0 0 0 2
K5–1 positive 50 0 0 0 50
free 0 0 0 0 0
K5–2 positive 14 1 0 0 15
free 0 0 0 0 0
K6–1 positive 12 3 0 0 15
free 0 0 0 0 0
  1. aThe letter and first number correspond to the number of the parental T0 plant