Table 2 Frequency of Cas9-positive and Cas9-free T2 seeds
T1 plant numbera | Cas9 integration | Number of T2 seeds | ||||
|---|---|---|---|---|---|---|
Mutation | No mutation | Total | ||||
Gly m Bd 28 K and Gly m Bd 30 K | Gly m Bd 28 K only | Gly m Bd 30 K only | ||||
E1–1 | positive | 16 | 10 | 3 | 0 | 29 |
free | 2 | 1 | 5 | 2 | 10 | |
E1–2 | positive | 21 | 0 | 0 | 0 | 21 |
free | 1 | 0 | 3 | 0 | 4 | |
E1–5 | positive | 8 | 4 | 0 | 1 | 13 |
free | 1 | 0 | 3 | 0 | 4 | |
E2–1 | positive | 0 | 20 | 0 | 5 | 25 |
free | 0 | 2 | 0 | 5 | 7 | |
E2–2 | positive | 1 | 20 | 0 | 0 | 21 |
free | 0 | 0 | 0 | 5 | 5 | |
K1–1 | positive | 9 | 1 | 0 | 0 | 10 |
free | 3 | 0 | 0 | 0 | 3 | |
K1–3 | positive | 13 | 3 | 0 | 0 | 16 |
free | 3 | 0 | 4 | 2 | 9 | |
K2–1 | positive | 16 | 3 | 1 | 0 | 20 |
free | 1 | 0 | 2 | 0 | 3 | |
K2–2 | positive | 19 | 7 | 0 | 0 | 26 |
free | 1 | 0 | 4 | 0 | 5 | |
K4–1 | positive | 33 | 0 | 2 | 0 | 35 |
free | 2 | 0 | 0 | 0 | 2 | |
K5–1 | positive | 50 | 0 | 0 | 0 | 50 |
free | 0 | 0 | 0 | 0 | 0 | |
K5–2 | positive | 14 | 1 | 0 | 0 | 15 |
free | 0 | 0 | 0 | 0 | 0 | |
K6–1 | positive | 12 | 3 | 0 | 0 | 15 |
free | 0 | 0 | 0 | 0 | 0 | |