Fig. 4
SDS-PAGE and immunoblot analyses of the crude proteins of representative double-mutant T3 and wild-type mature seeds. a Proteins separated by SDS-PAGE and stained with Coomasie Brilliant Blue. Designations of mutations are as in Table 3. Asterisks denote signal of putative Gly m Bd 30 K protein. M, molecular weight marker. b Immunoblot analysis using polyclonal antibody against Gly m Bd 28 K protein. c Immunoblot analysis using polyclonal antibody against Gly m Bd 30 K protein. Gly m Bd 30 K protein. Images of full-length gel and blots are provided in additional file 2 (Figure S8)